TOPOGRAPHIC VARIATION OF THE PROTEOGLYCAN POPULATION IN TWO DIFFERENT REGIONS OF CHICKEN TARSOMETARSAL CARTILAGE
DOI:
https://doi.org/10.24933/rep.v8i1.317Keywords:
Tecido conjuntivo; glicosaminoglicanos; variação anatômica.Abstract
The structural organization and distribution of proteoglycans in the extracellular matrix of the articular cartilage of young adult chickens were analyzed using samples from the lateral and medial surfaces of the tarsometatarsus. For structural analysis, the sections were stained with Alcian Blue and Xylidine-ponceau and analyzed by bright field and polarized light microscopies. For biochemical analysis, the components of the extracellular matrix were extracted with 4M guanidine hydrochloride, dosage, fractionated by gel filtration and ionic Exchange chromatographies and analyzed by electrophoresis on agarose, agarose-polyacrylamide and polyacrylamide supports. The structural analysis showed that the collagen fibrils were arranged transversally on the periphery of the two regions. The central area of the lateral region showed more intense reactivity to Alcian Blue. The uronic acid content of the lateral region was higher than the medial region. Analysis of glycosaminoglycans in agarose gels revealed the presence of only chondroitin sulfate. This showed no differences in relation to chain size. Analysis of large proteoglycans revealed larger molecules in the medial region. The electrophoretic bands corresponding to Mr of 230, 220, 180 and 59 kDa showed reactivity for Fibormodulim in both regions. The electrophoretic band corresponding to 75kDa showed reactivity for Decorim in both regions. The changes observed regarding tissue metachromasia and distribution of proteoglycans between the regions of the tarsometatarsal cartilage may be related to differences in the distribution of mechanical forces on the articular surface.
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BARNETT, C.H. A comparison of the human knee and avian ankle. Journal of Anatomy, v. 88, n. 1, p. 59 -70, 1954.
BLUM, B.; BEIER, H.; GROSS, H.J. Improved silver staining of plant proteins, RNA in polyacrylamide gels. Electrophoresis, v. 8, n. 1, p. 93-99, 1987.
BRADFORD, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principal of protein dye binding. Analytical Biochemistry, v. 72, n. 3, p. 248-254, 1976.
CAMILA B. CARBALLO, C. B.; NAKAGAWA, Y.; SEKIYA, I.; RODEO, S. A. Basic Science of Articular Cartilage. Clinics in Sports Medicine, v. 36, n. 3, p. 413-425, 2017.
CHEN, C.; TAMBE, D. T.; DENG, L.; YANG, L. Biomechanical properties and mechanobiology of the articular chondrocyte. American Journal of Physiology, v. 305, n. 12, p.1202 – 1208, 2013.
DIETRICH, C.P.; DIETRICH, S.M.C. Electrophoretic behaviour of acidic mucopolysaccharides in diamine buffers. Analytical Biochemistry, v. 70, n. 4, p. 645-647, 1976.
DISCHE, Z. A new specific color reaction of hexuronic acids. Journal Biological Chemistry, v. 167, n. 1, p. 189-199, 1947.
EGGLI, P.S.; HUNZIKER, E.B.; SCHENK, R.K. Quantification of structural features characterizing weight- and less- weigth-bearing ergions in articular cartilage: a steriological analysis of medical femoral condyles in young rabbits. Anatomical Records, v. 222, n. 3, p. 217-227, 1988.
ESQUISATTO, M.A.M.; PIMENTEL, E.R.; GOMES, L. Extracellular matrix composition of different regions of knee joint cartilage in cattle. Annals of Anatomy, v. 179, n.7, p. 433 – 437, 1997.
ESQUISATTO, M. A. M.; CONTRICIANI, R. E.; PIMENTEL, E. R.; GOMES, L. População de proteoglicanos em diferentes sítios anatômicos da cartilagem articular do joelho bovino. Ensaios Pioneiros, v. 7, n.1, p. 73 -89, 2023.
FARNDALE, R.W.; BUTTLE, D.J.; BARRET, A.J. Improved quantitation and discrimination of sulphated glyocosaminoglycans by use of dimethylmethylene blue. Biochemica Biophysica Acta, v. 883, n. 2, p. 173-177, 1986.
GOLDRING, M. B. Update on the biology of the chondrocyte and new approaches to treating cartilage diseases. Best Practice & Research Clinical Rheumatology, v. 20, n. 5, p. 1003 – 1025, 2006.
GOMES, L.; ESQUISATTO, M.A.M.; BELLINE, P.; PIMENTEL, E.R. Is there a relationship between the state of aggregation of small proteoglycans and the biomechanical properties of tissues? Brazilian Journal of Medical and Biological Research, v. 29, n. 10, p. 1243-1246, 1996.
HALL, A.C.; URBAN, J.P.G.; GEHL, K.A. The effects of hydrostatic pressure on matrix synthesis in articular cartilage. Journal Orthopaedical Research, v. 9, n. 1, p. 1-10, 1991.
JURVELIN, J.; HELMINEN, H. J.; LAURITSALO, S. Influences of joint immobilization and running exercise on articular cartilage surfaces of young rabbits. Acta Anatomica, v. 122, n. 1, p. 62- 68, 1985.
KHAN, M.; REDMAN, S. N.; WILLIAMS, R.; DOWTHWAITE, G. P.; OLDFIELD, S. F.; ARCHER, C. W. The Development of Synovial Joints. Current Topics in Developmental Biology, v.79, n. 1, p. 1 – 36, 2007.
KIM, Y.J.; SAH, R.L.Y.; GRODZINSKY, A.J.; PLAAS, A.H.K.; SANDY, J.D. Mechanical regulation of cartilage biosynthetic behaviour: physical stimuli. Archives of Biochemistry and Biophysics, v. 311, n. 1, p. 1-12, 1994.
KIVIRANTA, I.; SÖMANEN, A.M.; JURVELIN, J.S.; AROKOSKI, J.; OETTMEIER, R.; ABENDROTH, K.; ROTH, A.J.; TAMMI, M.I. Effect of motion and load on articular cartilage in animal models. In: KUETTNER, K.E.; SCHLEYERBACH, R.; PEYRON, J.G.; HASCALL, V.C. (eds). Articular Cartilage and Osteoarthritis. New York: Raven Press Ltd. p. 501-510, 1992.
LIN, Z.; WILLERS, C.; XU, J.; ZHENG, M. H. The chondrocyte: biology and clinical application. Tissue Engineering, v. 12, n. 7, p. 1971 – 1984, 2006.
LITTLE, C.B.; GHOSH, P. Variation in proteoglycan metabolism by articular chondrocytes in different joint regions is determined by post-natal mechanical loading. Osteoarthritis and Cartilage, v. 5, n. 1, p. 49 – 62, 1997.
LIU, J.; LAU, T.M.; CHOI, H.U.; TANG, L.H.; ROSENBERG, L.C. The self-association of biglycan from bovine articular cartilage. Journal of Biological Chemistry, v. 269, n. 14, p. 28366-28373, 1994.
MEYERS, R. A. Comparative anatomy of the postural mechanisms of the forelimbs of birds and mammals. Journal of Ornithology, v. 160, n.12, p. 869 – 882, 2019.
PALMOSKI, M.; PERRICONE, E.; BRANDT, K.D. Development and reversal of a proteoglycan aggregation defect in normal canine knee cartilage after immobilization. Arthritis and Rheumatism, v. 22, n. 10, p. 508-517, 1979.
ROBERTS, S.; WEIGHTMAN, B.; URBAN, J.P.G.; CHAPELL, D. Mechanical and biochemical properties of human articular cartilage in osteoarthritic femoral heads and in autopsy specimens. Journal of Bone and Joint Surgery, v. 68-B, n. 3, p. 278-288, 1986.
RODRIGUES, E. D.; PIMENTEL, E. R.; MOURÃO, P. A. S.; GOMES, L. Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens. Brazilian Journal of Medical and Biological Research v. 38, n. 4, p. 381-390, 2005.
SAH, R.; GRODZINKY, A.; PLAAS, A.H.; SANDY, J. Effects of static and dynamic compression on matrix metabolism in artilage explants. In: KUETTNER, K.E.; SCHLEYERBACH, R.; PEYRON, J.G.; HASCALL, V.C. (eds). Articular Cartilage and Osteoarthritis. New York: Raven Press Ltd. p. 373-392, 1992.
SCHNEIDERMAN, R.; KERET, D.; MAROUDAS, A. Effects of mechanical and osmotic-pressure on the rate of glycosaminoglycans synthesis in the human adult femoral head cartilage: an in vitro study. Journal Orthopaedical Research, v. 4, n. 8, p.393-408, 1986.
SCOTT, R. A.; PANITCH, A. Glycosaminoglycans in Biomedicine. Wiley Interdisciplinary Review of Nanomedicine and Nanobiotechnology, v. 5, n. 4, p. 388 – 398, 2013.
SLOWMAN, S.D.; BRANDT, K.D. Composition and glycosaminoglycan metabolism of articular cartilage from habitually loaded and habitually unload sites. Arthritis and Rheumatism, v. 29, n.1, p. 88-94, 1986.
SÖMANEN, A.M.; TAMMI, M.I.; KIVIRANTA, I.; JURVELIN, J.S.; HELMINEN, H.J. Maturation of proteoglycan matrix in articular cartilage under increased and decreased joint loading. Connective Tissue Research, v. 16, n. 3, p. 163 -175, 1987.
TOMIOSSO, T. C.; GOMES, L.; VIDAL, B. C.; PIMENTEL, E. R. Extracellular matrix of ostrich articular cartilage. Biocell, v. 29, n. 1, p. 47 – 54, 2005.
TOWBIN, H.; STAEHELIN, T.; GORDON J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of National Academy of Sciences – USA, v. 76, n. 9, p. 4350 – 4354, 1979.
ULRICH-VINTHER, M.; MALONEY, M. D.; SCHWARZ, E.M.; ROSIER, R.; REGIS J O'KEEFE, R. J. Articular cartilage biology. Journal American Academy of Orthopaedic Surgery, v. 11, n. 6, p. 421-430, 2003.
URBAN, J.P.G. The chondrocyte: a cell under pressure. British Journal of Rheumatology, v. 33, n. 10, p. 901- 908, 1994.
VISSER, N.A.; VANKAMPEN, G.P.; DEKONING, M.H.; VANDERKORST, J.K. The effects of loaging on the synthesis of biglycan and decorin in intact mature articular cartilage in vitro. Connective Tissue Research, v. 30, n. 3, p. 241- 250, 1994.
ZINGALES, B. (1994). Analysis of protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In: Genes and Antigens of Parasites, edited by MOREL, C.M., Fiocruz, Rio de Janeiro.
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